Tomlinson C R
Mutat Res. 1980 Apr;70(2):179-91. doi: 10.1016/0027-5107(80)90158-x.
Sodium azide at various pH values did not cause a significant increase in the frequency of forward mutation above the control frequency at the adenine-3 (ad-3) region in resting conidia and in conidia from growing cultures of heterokaryons 12 and 59 of Neurospora crassa. Conidia from ad-3 mutants were plated with sodium azide at various pH values, and no obvious increase in reverse mutation above the controls was observed. Data are presented showing that sodium azide at pH 3 is inactivating conidia by interacting with the cytoplasma rather than the nucleus, and this may be the primary reasons that no mutation at the ad-3 region was detected. The dependence of sodium azide mutagenicity on pH was investigated in histidine-requiring mutants of Salmonella typhimurium using a suspension test. There were no significant differences in the reversion frequencies among the pH values (3-8) tested. Thus, no pH dependence is associated with sodium azide mutagenicity, nor are growth and/or DNA replication required for mutagenicity by sodium azide, in S. typhimurium.
在不同pH值下，叠氮化钠并未使静止分生孢子以及粗糙脉孢菌异核体12和59生长培养物中的分生孢子在腺嘌呤-3（ad-3）区域的正向突变频率显著高于对照频率。将来自ad-3突变体的分生孢子与不同pH值的叠氮化钠一起接种，未观察到回复突变明显高于对照。所呈现的数据表明，pH值为3时的叠氮化钠通过与细胞质而非细胞核相互作用使分生孢子失活，这可能是未检测到ad-3区域发生突变的主要原因。使用悬浮试验在鼠伤寒沙门氏菌的组氨酸需求突变体中研究了叠氮化钠诱变性对pH的依赖性。在所测试的pH值（3 - 8）之间，回复频率没有显著差异。因此，在鼠伤寒沙门氏菌中，叠氮化钠的诱变性与pH无关，叠氮化钠诱变性也不需要生长和/或DNA复制。
