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痘苗病毒DNA中的反向末端重复序列编码早期mRNA。

Inverted terminal repetition in vaccinia virus DNA encodes early mRNAs.

作者信息

Wittek R, Barbosa E, Cooper J A, Garon C F, Chan H, Moss B

出版信息

Nature. 1980 May 1;285(5759):21-5. doi: 10.1038/285021a0.

DOI:10.1038/285021a0
PMID:6445508
Abstract

Vaccinia virus DNA contains a long inverted terminal repetition of MW approximately 6.8 x 10(6). A fragment of MW 6.3 X 10(6) from this repetition has been cloned in coliphage lambda and used to isolate RNA from virus-infected cells. Electron microscopy indicates that early RNAs are transcribed from the repeated sequence and cell-free translation shows that the RNAs code for polypeptides.

摘要

痘苗病毒DNA含有一个分子量约为6.8×10⁶的长反向末端重复序列。从该重复序列中分离出一个分子量为6.3×10⁶的片段,克隆到大肠杆菌噬菌体λ中,并用于从病毒感染细胞中分离RNA。电子显微镜检查表明,早期RNA是从重复序列转录而来的,无细胞翻译表明这些RNA编码多肽。

相似文献

1
Inverted terminal repetition in vaccinia virus DNA encodes early mRNAs.痘苗病毒DNA中的反向末端重复序列编码早期mRNA。
Nature. 1980 May 1;285(5759):21-5. doi: 10.1038/285021a0.
2
Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.痘苗病毒基因组反向末端重复序列内编码的mRNA的杂交选择和无细胞翻译。
J Virol. 1981 Jan;37(1):284-94. doi: 10.1128/JVI.37.1.284-294.1981.
3
Transcriptional and translational mapping of a 6.6-kilobase-pair DNA fragment containing the junction of the terminal repetition and unique sequence at the left end of the vaccinia virus genome.对一个6.6千碱基对DNA片段进行转录和翻译图谱分析,该片段包含痘苗病毒基因组左端末端重复序列与独特序列的连接处。
J Virol. 1981 Sep;39(3):722-32. doi: 10.1128/JVI.39.3.722-732.1981.
4
Expression of the vaccinia virus genome: analysis and mapping of mRNAs encoded within the inverted terminal repetition.痘苗病毒基因组的表达:对反向末端重复序列内编码的mRNA的分析与定位
Cell. 1980 Sep;21(2):487-93. doi: 10.1016/0092-8674(80)90485-7.
5
Colinearity of RNAs with the vaccinia virus genome: anomalies with two complementary early and late RNAs result from a small deletion or rearrangement within the inverted terminal repetition.RNA与痘苗病毒基因组的共线性:两条互补的早期和晚期RNA的异常是由反向末端重复序列内的小缺失或重排导致的。
J Virol. 1982 May;42(2):447-55. doi: 10.1128/JVI.42.2.447-455.1982.
6
Complete nucleotide sequences of two adjacent early vaccinia virus genes located within the inverted terminal repetition.位于反向末端重复序列内的两个相邻痘苗病毒早期基因的完整核苷酸序列。
J Virol. 1982 Nov;44(2):637-46. doi: 10.1128/JVI.44.2.637-646.1982.
7
DNA-dependent RNA polymerase subunits encoded within the vaccinia virus genome.痘苗病毒基因组内编码的依赖DNA的RNA聚合酶亚基。
J Virol. 1987 Jun;61(6):1765-71. doi: 10.1128/JVI.61.6.1765-1771.1987.
8
Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment.通过对痘苗病毒HindIII H基因组片段的结构和功能分析推断出的痘苗病毒晚期基因转录和翻译起始位点处保守的TAAATG序列。
J Virol. 1986 Nov;60(2):436-49. doi: 10.1128/JVI.60.2.436-449.1986.
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Transcriptional and translational analysis of a strongly expressed early region of the vaccinia virus genome.痘苗病毒基因组一个高表达早期区域的转录和翻译分析
J Virol. 1984 Feb;49(2):459-70. doi: 10.1128/JVI.49.2.459-470.1984.
10
Deletion of a 9,000-base-pair segment of the vaccinia virus genome that encodes nonessential polypeptides.删除痘苗病毒基因组中一段编码非必需多肽的9000个碱基对的片段。
J Virol. 1981 Nov;40(2):387-95. doi: 10.1128/JVI.40.2.387-395.1981.

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Vaccine. 2013 Sep 6;31(39):4220-2. doi: 10.1016/j.vaccine.2013.03.042. Epub 2013 Apr 10.
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Dinucleotide Sequences at the 5' Ends of Vaccinia Virus mRNA's Synthesized In Vitro.
J Virol. 1980 Nov;36(2):601-5. doi: 10.1128/JVI.36.2.601-605.1980.
4
Complete nucleotide sequences of two adjacent early vaccinia virus genes located within the inverted terminal repetition.位于反向末端重复序列内的两个相邻痘苗病毒早期基因的完整核苷酸序列。
J Virol. 1982 Nov;44(2):637-46. doi: 10.1128/JVI.44.2.637-646.1982.
5
Colinearity of RNAs with the vaccinia virus genome: anomalies with two complementary early and late RNAs result from a small deletion or rearrangement within the inverted terminal repetition.RNA与痘苗病毒基因组的共线性:两条互补的早期和晚期RNA的异常是由反向末端重复序列内的小缺失或重排导致的。
J Virol. 1982 May;42(2):447-55. doi: 10.1128/JVI.42.2.447-455.1982.
6
Vaccinia virus RNA polymerase associated with nuclei of infected HeLa cells.与受感染的HeLa细胞核相关的痘苗病毒RNA聚合酶。
J Virol. 1984 Jan;49(1):26-34. doi: 10.1128/JVI.49.1.26-34.1984.
7
In vitro transcription of the inverted terminal repetition of the vaccinia virus genome: correspondence of initiation and cap sites.痘苗病毒基因组反向末端重复序列的体外转录:起始位点与帽位点的对应关系。
J Virol. 1981 Feb;37(2):738-47. doi: 10.1128/JVI.37.2.738-747.1981.
8
Hybridization selection and cell-free translation of mRNA's encoded within the inverted terminal repetition of the vaccinia virus genome.痘苗病毒基因组反向末端重复序列内编码的mRNA的杂交选择和无细胞翻译。
J Virol. 1981 Jan;37(1):284-94. doi: 10.1128/JVI.37.1.284-294.1981.
9
Transcriptional and translational analysis of a strongly expressed early region of the vaccinia virus genome.痘苗病毒基因组一个高表达早期区域的转录和翻译分析
J Virol. 1984 Feb;49(2):459-70. doi: 10.1128/JVI.49.2.459-470.1984.
10
Marker rescue of temperature-sensitive mutations of vaccinia virus WR: correlation of genetic and physical maps.痘苗病毒WR温度敏感突变的标志物拯救:遗传图谱与物理图谱的相关性
J Virol. 1983 Nov;48(2):419-28. doi: 10.1128/JVI.48.2.419-428.1983.