Moss Bernard
From the Laboratory of Viral Diseases, NIAID, National Institutes of Health, Bethesda, Maryland 20892
J Biol Chem. 2017 Mar 10;292(10):3958-3969. doi: 10.1074/jbc.X117.778712. Epub 2017 Jan 30.
This Reflections article describes my early work on viral enzymes and the discovery of mRNA capping, how my training in medicine and biochemistry merged as I evolved into a virologist, the development of viruses as vaccine vectors, and how scientific and technological developments during the 1970s and beyond set the stage for the interrogation of nearly every step in the reproductive cycle of vaccinia virus (VACV), a large DNA virus with about 200 genes. The reader may view this article as a work in progress, because I remain actively engaged in research at the National Institutes of Health (NIH) notwithstanding 50 memorable years there.
这篇反思文章描述了我早期在病毒酶方面的工作以及mRNA加帽的发现，讲述了我在医学和生物化学方面的训练如何在我成长为一名病毒学家的过程中融合在一起，病毒作为疫苗载体的发展，以及20世纪70年代及以后的科学技术发展如何为研究痘苗病毒（VACV）——一种拥有约200个基因的大型DNA病毒——复制周期中的几乎每一步奠定了基础。读者可以将本文视为一篇仍在完善的作品，因为尽管我在国立卫生研究院（NIH）度过了50个难忘的年头，但我仍在那里积极从事研究工作。
