Gerrard J M, Stuart M J, Rao G H, Steffes M W, Mauer S M, Brown D M, White J G
J Lab Clin Med. 1980 Jun;95(6):950-8.
An evaluation of platelet and vascular (aortic) arachidonic acid metabolism was performed in Lewis male rats rendered diabetic by injection of streptozotocin, and the results were compared to those in matched controls. Parameters evaluated included the release of this fatty acid from prelabeled platelets and aortas and conversion of labeled fatty acid to thromboxane B2 and 6-keto-PGF1 alpha in platelets and aortas, respectively. Diabetic rat platelets showed markedly increased release of arachidonic acid with thrombin used as the aggregating stimulus. Conversion of arachidonic acid to thromboxane B2 was slightly, but not significantly, higher in the diabetic rats. In the vessel, thrombin-stimulated release of arachidonic acid was slightly, but not significantly, increased in the diabetic animals when compared to controls. This finding was associated with a decrease in vascular production of 6-keto-PGF1 alpha both in vascular tissues incubated with arachidonic acid alone and in vascular tissues incubated with thrombin. The changes observed both in platelet and vascular metabolism of arachidonic acid were corrected by islet issue transplantation, suggesting a disease-specific effect. The changes observed in arachidonic acid metabolism suggest a significant imbalance in thromboxane A2 and PGI2 production in diabetic rats. Such changes might promote the development of the microvascular changes seen in diabetes mellitus.
对通过注射链脲佐菌素诱导为糖尿病的雄性Lewis大鼠的血小板和血管（主动脉）花生四烯酸代谢进行了评估，并将结果与匹配的对照组进行了比较。评估的参数包括预先标记的血小板和主动脉中这种脂肪酸的释放，以及分别在血小板和主动脉中标记脂肪酸向血栓素B2和6-酮-前列环素F1α的转化。糖尿病大鼠血小板在以凝血酶作为聚集刺激物时，花生四烯酸的释放明显增加。糖尿病大鼠中花生四烯酸向血栓素B2的转化略有升高，但无统计学显著差异。在血管中，与对照组相比，糖尿病动物中凝血酶刺激的花生四烯酸释放略有增加，但无统计学显著差异。这一发现与单独用花生四烯酸孵育的血管组织以及用凝血酶孵育的血管组织中血管6-酮-前列环素F1α生成的减少有关。在血小板和血管花生四烯酸代谢中观察到的变化通过胰岛移植得到纠正，提示存在疾病特异性效应。在花生四烯酸代谢中观察到的变化表明糖尿病大鼠中血栓素A2和前列环素I2生成存在显著失衡。此类变化可能促进糖尿病中所见微血管变化的发展。
