Stuart M J, Gerrard J M, White J G
N Engl J Med. 1980 Jan 3;302(1):6-10. doi: 10.1056/NEJM198001033020102.
We altered platelet cholesterol by incubating the cells with either "cholesterol-rich" or "cholesterol-poor" liposomes. These platelets were then used to study the influence of cholesterol content on the metabolism of arachidonic acid, a fatty acid that serves as the critical precursor in the platelet for formation of the potent aggregating agent thromboxane A2. After addition of the aggregating agent thrombin, cholesterol-enriched platelets released 18.1 +/- 0.6 per cent (mean +/- 1 S.E.M.) [14C]arachidonic acid from prelabeled platelet phospholipids. This value was higher (P less than 0.001) than that for cholesterol-depleted platelets (14.6 +/- 1.0 per cent). Conversion of released arachidonic acid to platelet thromboxane B2 (the stable end product of thromboxane A2) was also higher in cholesterol-rich platelets (22.6 +/- 3.9 per cent) than in cholesterol-depleted platelets (13.8 +/- 2.7 per cent). These studies show that changes in the cholesterol content of human platelets in vitro have a significant effect on platelt metabolism of arachidonic acid. N Engl J Med 302:6-10, 1980).
我们通过将细胞与“富含胆固醇”或“胆固醇含量低”的脂质体一起孵育来改变血小板胆固醇含量。然后用这些血小板来研究胆固醇含量对花生四烯酸代谢的影响，花生四烯酸是血小板中强效聚集剂血栓素A2形成的关键前体脂肪酸。加入聚集剂凝血酶后，胆固醇含量升高的血小板从预先标记的血小板磷脂中释放出18.1±0.6%（平均值±1个标准误）的[14C]花生四烯酸。该值高于胆固醇含量降低的血小板（14.6±1.0%）（P<0.001）。释放的花生四烯酸转化为血小板血栓素B2（血栓素A2的稳定终产物）在胆固醇含量高的血小板中（22.6±3.9%）也高于胆固醇含量降低的血小板（13.8±2.7%）。这些研究表明，体外人血小板胆固醇含量的变化对花生四烯酸的血小板代谢有显著影响。（《新英格兰医学杂志》302:6 - 10, 1980年）
