Hanson B A, Lester R L
J Lipid Res. 1980 Mar;21(3):309-15.
By use of fungi grown in the presence of [3H]-inositol and [14C]choline, we have explored methods for the quantitative extraction of inositol-containing phospholipids and phosphatidylcholine. Slightly alkaline mixtures of both ethanol-water and ethanol-diethylether-water at elevated temperatures were shown to effectively extract these lipids from intact Saccharomyces cerevisiae and Neurospora crassa. Some previously published procedures fail to completely extract to very polar phosphoinositol-containing sphingolipids of these organisms. Trichloroacetic acid can be used with caution in killing cells prior to extraction; lipid destruction can occur at elevated concentrations and temperatures. Complete extraction of these very polar lipids with polar solvents also results in an extract containing significant amounts of non-lipids.
通过使用在[3H]-肌醇和[14C]胆碱存在下生长的真菌，我们探索了定量提取含肌醇磷脂和磷脂酰胆碱的方法。结果表明，在升高的温度下，乙醇-水和乙醇-二乙醚-水的微碱性混合物能有效地从完整的酿酒酵母和粗糙脉孢菌中提取这些脂质。一些先前发表的方法无法完全提取这些生物体中极性很强的含磷酸肌醇鞘脂。在提取前，可以谨慎使用三氯乙酸杀死细胞；在较高浓度和温度下可能会发生脂质破坏。用极性溶剂完全提取这些极性很强的脂质也会得到含有大量非脂质的提取物。
