粗糙脉孢菌5.8S核糖体核糖核酸的特性分析
Characterization of 5.8S ribosomal ribonucleic acid in Neurospora crassa.
作者信息
Lucas M C, Vanderhoef L N
出版信息
J Bacteriol. 1975 Nov;124(2):736-9. doi: 10.1128/jb.124.2.736-739.1975.
Abstract
Neurospora crassa ribosomes contain a species of ribonucleic acid (RNA) of molecular weight 54,000, similar to 5.8S ribosomal RNA previously described for other eukaryotic organisms. The 5.8S RNA from N. crassa was found to be released by heat treatment at 60 C from 25S ribosomal RNA but not from 18S ribosomal RNA. The base composition of N. crassa 5.8S RNA was similar to that of 5.8S RNA from Saccharomyces cerevisiae, but differed from animal 5.8S RNA. During the course of this study, it was discovered that N. crassa 25S ribosomal RNA had a number of internal cleavages that may exist in vivo.
摘要
粗糙脉孢菌核糖体含有一种分子量为54,000的核糖核酸（RNA），类似于先前在其他真核生物中描述的5.8S核糖体RNA。发现来自粗糙脉孢菌的5.8S RNA在60℃热处理时从25S核糖体RNA中释放出来，但不从18S核糖体RNA中释放。粗糙脉孢菌5.8S RNA的碱基组成与酿酒酵母5.8S RNA的碱基组成相似，但与动物5.8S RNA不同。在这项研究过程中，发现粗糙脉孢菌25S核糖体RNA有许多可能在体内存在的内部切割。
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