Simek J, Cervinková Z, Smejkalová J, Sobotka L, Chmelar V
Physiol Bohemoslov. 1980;29(2):161-6.
The authors studied the effect of realimentation after several days' isolated glucose or fructose intake on DNA synthesis in liver regenerating after partial hepatectomy (PH) (65-70%) or after carbon tetrachloride (CCl4) poisoning 1.5 ml/kg. Two days before PH or the administration of CCl4 and two days after, the experimental rats were given glucose (50% solution) of fructose (50% solution) as the only source of energy. Rats with PH were then fed for one day on a standard laboratory diet (25 cal% protein) or a high protein diet (81 cal% protein). Rats with CCl4 liver damage were fed for one day on the standard laboratory diet only. In the rats given glucose, liver DNA synthesis and the total amount of these nucleic acids in the liver 48 hours after CCl4 administration was lower than in the controls or the rats given fructose. In all the experimental groups (PH and CCl4), stimulation of liver DNA synthesis was observed after one day's realimentation. The total DNA content of the liver of rats with PH rose markedly during realimentation. The experiments indicate that the regenerative activity of damaged liver can be influenced by the nutritional regimen.
作者研究了在部分肝切除（PH）（65 - 70%）或四氯化碳（CCl4）以1.5 ml/kg剂量中毒后，连续数天单独摄入葡萄糖或果糖后再给予营养补充对肝脏再生过程中DNA合成的影响。在PH手术或给予CCl4之前两天以及之后两天，给实验大鼠喂食葡萄糖（50%溶液）或果糖（50%溶液）作为唯一能量来源。然后，对接受PH手术的大鼠喂食标准实验室饮食（蛋白质含量为25 cal%）或高蛋白饮食（蛋白质含量为81 cal%）一天。对CCl4肝损伤大鼠仅喂食标准实验室饮食一天。在给予葡萄糖的大鼠中，CCl4给药48小时后肝脏DNA合成及肝脏中这些核酸的总量低于对照组或给予果糖的大鼠。在所有实验组（PH组和CCl4组）中，给予一天营养补充后均观察到肝脏DNA合成受到刺激。接受PH手术的大鼠在营养补充期间肝脏的总DNA含量显著升高。这些实验表明，受损肝脏的再生活性可受到营养方案的影响。
