Kanta J, Chmelar V
Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove. 1989;32(4):395-401.
Rat liver DNA was labelled with [methyl-3H] thymidine after partial hepatectomy, carbon tetrachloride poisoning, or an intravenous injection of silica dust. Changes in DNA labelling were studied for 4 weeks after the single pulse. Total radioactivity incorporated into liver DNA after partial hepatectomy and after carbon tetrachloride administration remained on the same level when compared with that found after 1 h. DNA activity in liver of untreated rats and of rats treated with silica decreased by about 50% within the first 2 weeks and then remained on this level for the rest of the studied period. These differences may reflect the fact that hepatocytes that have a long life span are preferentially labelled in partially hepatectomized and CCl4-treated rats, while liver macrophages with a short half-life take up a large part of the label in intact rats and in rats treated with silica.
在部分肝切除、四氯化碳中毒或静脉注射二氧化硅粉尘后，用[甲基-³H]胸苷标记大鼠肝脏DNA。单次脉冲后4周研究DNA标记的变化。与1小时后相比，部分肝切除和给予四氯化碳后掺入肝脏DNA的总放射性保持在同一水平。未处理大鼠和用二氧化硅处理的大鼠肝脏中的DNA活性在前2周内下降约50%，然后在研究期的其余时间保持在该水平。这些差异可能反映了这样一个事实，即在部分肝切除和CCl₄处理的大鼠中，寿命长的肝细胞被优先标记，而半衰期短的肝巨噬细胞在完整大鼠和用二氧化硅处理的大鼠中摄取了大部分标记物。
