Leal J J, Finlayson B
Invest Urol. 1977 Jan;14(4):278-83.
The adsorption of proteins and mucopolysaccharides on calcium oxalate crystals was measured by solution depletion. Anionic protein adsorption was found to be sensitive to calcium ion concentration. Adsorption of fibrinogen was anomalously large in the presence of 0.01 M Ca2+. Adsorption of cationic protein (histone) was sensitive to oxalate ion concentration. A small alteration in adsorption of protein as a result of pH or temperature change was also observed. Plots of adsorption versus concentration were interpreted in terms of a Langmuir adsorption isotherm. The derived Langmuir adsorption parameters were then used to investigate the contribution of protein, by physical adsorpti, to the quantity of matrix in urinary stones. It was concluded that physical adsorption can account for the deposition of part but not all of the matrix in calcium oxalate stones. It was also concluded that physical adsorption of mucopolysaccharides by calcium oxalate crystals could explain the inhibition of growth and aggregation of calcium oxalate crystals seen with in vitro precipitation systems. Recalculation of published data indicates that adsorption of protein on dental enamel (calcium hydroxyapatite) results in approximately the same extent of surface coverage as adsorption on calcium oxalate crystals, but protein has a much lower affinity for dental enamel than for calcium oxalate crystals.
通过溶液消耗法测定蛋白质和粘多糖在草酸钙晶体上的吸附情况。发现阴离子蛋白吸附对钙离子浓度敏感。在0.01 M Ca2+存在下，纤维蛋白原的吸附异常大。阳离子蛋白（组蛋白）的吸附对草酸根离子浓度敏感。还观察到由于pH或温度变化导致的蛋白质吸附的微小改变。吸附量与浓度的关系图根据朗缪尔吸附等温线进行解释。然后，将导出的朗缪尔吸附参数用于研究通过物理吸附作用的蛋白质对尿结石中基质含量的贡献。得出的结论是，物理吸附可以解释草酸钙结石中部分而非全部基质的沉积。还得出结论，草酸钙晶体对粘多糖的物理吸附可以解释体外沉淀系统中观察到的草酸钙晶体生长和聚集的抑制现象。对已发表数据的重新计算表明，蛋白质在牙釉质（羟基磷灰石钙）上的吸附导致的表面覆盖程度与在草酸钙晶体上的吸附大致相同，但蛋白质对牙釉质的亲和力比对草酸钙晶体的亲和力低得多。
