Ratanaubolchai K, Panijpan B
Int J Vitam Nutr Res. 1980;50(1):3-9.
The reaction between thiamine (37 micro M) and polyphenols (74 micro M) at 60 degrees, pH 7.4, is without an extremely rapid initial phase. The time-course of reaction is hyperbolic and the log [thiamine] vs time plots pseudo-first order with respect to thiamine. A tentative mechanism is proposed for the reaction after considering results with free radical scavengers and reducing agents together with previous data. As a by-product of the study, CNBr emerges as a better reagent than HgCl2 and K3Fe(CN)6 for thiochrome formation in the presence of polyphenols and reducing agents.
硫胺素（37微摩尔）与多酚（74微摩尔）在60摄氏度、pH值7.4条件下的反应没有极快速的初始阶段。反应的时间进程呈双曲线，并且硫胺素的对数[硫胺素]与时间的关系图对于硫胺素而言呈准一级反应。在结合自由基清除剂和还原剂的结果以及先前数据进行考虑之后，提出了该反应的一个初步机制。作为该研究的一个副产品，在多酚和还原剂存在的情况下，对于硫色素的形成而言，溴化氰是比氯化汞和铁氰化钾更好的试剂。
