Brunner A, Tuena de Cobos A
Mol Gen Genet. 1980;178(2):351-5. doi: 10.1007/BF00270483.
The mitochondrial ATPase from oligomycin-resistant mutants which map on different regions of an extrachromosomal DNA (01 and 011 class mutants) showed an increased resistance to oligomycin and venturicidin when assayed in vitro as compared to the sensitive strains. The resistance to oligomycin of the isolated mitochondrial ATPase from 01 class mutants was higher than that of the 011 class mutants. Cross resistance of the oligomycin-resistant mutants to the antibiotics peliomycin and ossamycin, which also inhibit phosphoryl transfer reactions in mitochondria (Walter et al., 1967), was observed, 01 mutants being more resistant to ossamycin than 011 class mutants. At the concentrations of peliomycin studied, no difference in sensitivity among both groups of oligomycin-resistant mutants could be detected. Mitochondrial respiration and isolated mitochondrial ATPase activity are sensitive to venturicidin, suggesting that the previously observed (Brunner et al., 1977) in vivo venturicidin resistance of K. lactis is probably due to an impairment of the influx of the drug at the level of the plasma membrane.
对位于一条染色体外DNA不同区域的抗寡霉素突变体（01和011类突变体）的线粒体ATP酶进行体外测定时，与敏感菌株相比，其对寡霉素和抗霉素A的抗性增强。从01类突变体中分离得到的线粒体ATP酶对寡霉素的抗性高于011类突变体。观察到抗寡霉素突变体对同样抑制线粒体磷酸转移反应的抗生素佩利霉素和奥沙霉素有交叉抗性（Walter等人，1967），01突变体对奥沙霉素的抗性比011类突变体更强。在所研究的佩利霉素浓度下，两组抗寡霉素突变体之间未检测到敏感性差异。线粒体呼吸作用和分离得到的线粒体ATP酶活性对抗霉素A敏感，这表明先前观察到的乳酸克鲁维酵母体内抗霉素A抗性（Brunner等人，1977）可能是由于药物在质膜水平的流入受损所致。
