文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Morpurgo L, Graziani M T, Finazzi-Agrò A, Rotilio G, Mondovì B

Biochem J. 1980 May 1;187(2):361-6. doi: 10.1042/bj1870361.

Spectroscopic and functional properties of Japanese-lacquer-tree (Rhus vernicifera) laccase were re-investigated, with special emphasis on the relationships between the different types of copper centres (Types 1, 2, and 3). 2. On removal of the Type 2 Cu(II), a decrease of absorbance occurred in the wavelength region above 650 nm (delta epsilon 750 = 300 M-1 . cm-1) and around 330 nm (delta episom 330 up to 2200 M-1 . cm-1). 3. Reductive titrations with ascorbic acid or ferrocyanide showed that the electron-accepting capacity of the partial apoprotein is one electron-equivalent lower than that of the native protein, i.e. the protein two-electron acceptor is present in the oxidized state in spite of absorbance loss at 330 nm. 4. The 330 nm chromophore apparently depends on the presence of both the Type 2 and the Type 3 copper in the oxidized state. 5. This finding may have implications in the relative location of Type 2 and 3 copper centres and on the redox behaviour of laccase.

对漆树漆酶的光谱和功能特性进行了重新研究，特别强调了不同类型铜中心（1型、2型和3型）之间的关系。2. 去除2型Cu(II)后，在650 nm以上波长区域（Δε750 = 300 M-1·cm-1）和330 nm左右（Δε330高达2200 M-1·cm-1）吸光度降低。3. 用抗坏血酸或亚铁氰化物进行的还原滴定表明，部分脱辅基蛋白的电子接受能力比天然蛋白低一个电子当量，即尽管在330 nm处吸光度下降，但蛋白质双电子受体仍以氧化态存在。4. 330 nm发色团显然取决于氧化态的2型和3型铜的同时存在。5. 这一发现可能对2型和3型铜中心的相对位置以及漆酶的氧化还原行为有影响。

Morpurgo L, Graziani M T, Finazzi-Agrò A, Rotilio G, Mondovì B

Biochem J. 1980 May 1;187(2):361-6. doi: 10.1042/bj1870361.

Spectroscopic and functional properties of Japanese-lacquer-tree (Rhus vernicifera) laccase were re-investigated, with special emphasis on the relationships between the different types of copper centres (Types 1, 2, and 3). 2. On removal of the Type 2 Cu(II), a decrease of absorbance occurred in the wavelength region above 650 nm (delta epsilon 750 = 300 M-1 . cm-1) and around 330 nm (delta episom 330 up to 2200 M-1 . cm-1). 3. Reductive titrations with ascorbic acid or ferrocyanide showed that the electron-accepting capacity of the partial apoprotein is one electron-equivalent lower than that of the native protein, i.e. the protein two-electron acceptor is present in the oxidized state in spite of absorbance loss at 330 nm. 4. The 330 nm chromophore apparently depends on the presence of both the Type 2 and the Type 3 copper in the oxidized state. 5. This finding may have implications in the relative location of Type 2 and 3 copper centres and on the redox behaviour of laccase.

对漆树漆酶的光谱和功能特性进行了重新研究，特别强调了不同类型铜中心（1型、2型和3型）之间的关系。2. 去除2型Cu(II)后，在650 nm以上波长区域（Δε750 = 300 M-1·cm-1）和330 nm左右（Δε330高达2200 M-1·cm-1）吸光度降低。3. 用抗坏血酸或亚铁氰化物进行的还原滴定表明，部分脱辅基蛋白的电子接受能力比天然蛋白低一个电子当量，即尽管在330 nm处吸光度下降，但蛋白质双电子受体仍以氧化态存在。4. 330 nm发色团显然取决于氧化态的2型和3型铜的同时存在。5. 这一发现可能对2型和3型铜中心的相对位置以及漆酶的氧化还原行为有影响。