噬菌体T4中的区域特异性重组。II. 重组体的结构。
Region-specific recombination in phage T4. II. Structure of the recombinants.
作者信息
Levy J N, Goldberg E B
出版信息
Genetics. 1980 Mar;94(3):531-47. doi: 10.1093/genetics/94.3.531.
In this paper, we present results of crosses designed to elucidate the structure of recombinants in the tail-fiber region of bacteriophage T4, in which a glucosylation-dependent recombinations mechanism is operative, and the cause of the "special" recombination in glycosylated crosses is discussed. We present evidence that, when phage are nonglycosylated, recombination in the tail-fiber region proceeds via long heteroduplex overlaps. Mismatched bases within such regions (in nonglycosylated phage) are repaired efficiently (as contrasted to those of glucosylated phage), but asymmetrically; that is, there may be an equal probability of resolving the mismatch to mutant or wild type.
在本文中，我们展示了为阐明噬菌体T4尾丝区域重组体结构而设计的杂交实验结果，其中一种糖基化依赖性重组机制起作用，并讨论了糖基化杂交中“特殊”重组的原因。我们提供的证据表明，当噬菌体未糖基化时，尾丝区域的重组通过长异源双链重叠进行。此类区域（未糖基化噬菌体中）内的错配碱基能被高效修复（与糖基化噬菌体的错配碱基形成对比），但修复过程是不对称的；也就是说，将错配碱基解析为突变型或野生型的概率可能相等。
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