Berndtson W E, Foote R H
J Dairy Sci. 1976 Dec;59(12):2130-3. doi: 10.3168/jds.S0022-0302(76)84498-0.
Motility of spermatozoa stored at 5 C with up to 1000 units or mug of chloramphenicol, polymyxin, kanamycin, tylosin, ampicillin, lincomycin, spectinomycin, erythromycin, novabiocin, or terramycin per ml of extender was compared to that with penicillin plus dihydrostreptomycin. Novabiocin and terramycin were toxic, but other antibiotic treatments had no effect. However, erythromycin and tylosin, as well as colymycin, depressed motility of frozen thawed spermatozoa. Spermatozoal motility was equivalent, following freezing in ampules or straws. All of the antibiotics which were non-toxic when added singly to frozen semen were also not harmful to frozen spermatozoa when as much as 2000 units or mug were added per ml of extender containing penicillin and dihydrostreptomycin. The addition of 1000 units or mug of ampicillin, chloramphenicol, kanamycin, lincomycin, polymyxin, or spectinomycin per ml of extender containing 750 units penicillin and 750 mug dihydrostreptomycin per ml did not influence the fertility of frozen spermatozoa in a field test involving 19,663 first inseminations.
将每毫升稀释液中添加多达1000单位或微克氯霉素、多粘菌素、卡那霉素、泰乐菌素、氨苄青霉素、林可霉素、壮观霉素、红霉素、新生霉素或土霉素的情况下，保存在5℃的精子活力与添加青霉素加双氢链霉素的情况进行了比较。新生霉素和土霉素有毒，但其他抗生素处理没有效果。然而，红霉素、泰乐菌素以及粘菌素会降低冻融精子的活力。安瓿或细管冷冻后精子活力相当。当每毫升含青霉素和双氢链霉素的稀释液中添加多达2000单位或微克时，所有单独添加到冷冻精液中无毒的抗生素对冷冻精子也无害。在一项涉及19663次首次输精的田间试验中，每毫升含750单位青霉素和750微克双氢链霉素的稀释液中添加1000单位或微克氨苄青霉素、氯霉素、卡那霉素、林可霉素、多粘菌素或壮观霉素，对冷冻精子的生育力没有影响。
