Stoianov T
Vet Med Nauki. 1987;24(3):57-66.
The effect was tested under laboratory in vitro conditions of eight antibiotics in media for thawing pelleted bull semen. It was found that the addition of antibiotics during the process of reanimation had no unfavourable impact on the motility of spermatozoa. Upon storage in water bath (39 degrees C) in the course of six hours gentamycin, amoxicillin, kanamycin, and polymixin preserved the semen cells and enhanced their survival rate at the same level as that of the control medium with no antibiotic. The combination of penicillin and streptomycin lowered 2.5 times spermatozoal motility. Worst was the survival rate of spermatozoa when the medium was supplemented with oxytetracycline and chloramphenicol. It was also found that best sanitation qualities with regard to the microflora in semen had gentamycin, which, at that did not produce any negative effect on the rate of survival. The conception rate of cows inseminated with semen treated with gentamycin at thawing was shown to be 15.3 per cent higher, and when treated with the combination of penicillin and streptomycin it was 3.0 per cent higher at first insemination than that of the control group of animals inseminated with semen with no antibiotic supplement.
在实验室体外条件下，对解冻颗粒公牛精液的培养基中的八种抗生素进行了效果测试。发现在复苏过程中添加抗生素对精子活力没有不利影响。在39摄氏度的水浴中储存六小时后，庆大霉素、阿莫西林、卡那霉素和多粘菌素能保存精液细胞，并使其存活率提高到与不含抗生素的对照培养基相同的水平。青霉素和链霉素的组合使精子活力降低了2.5倍。当培养基中添加土霉素和氯霉素时，精子的存活率最差。还发现，在精液微生物群方面，卫生质量最佳的是庆大霉素，它对存活率没有产生任何负面影响。结果表明，解冻时用庆大霉素处理的精液进行人工授精的母牛受胎率比未添加抗生素精液的对照组动物高15.3%，首次人工授精时用青霉素和链霉素组合处理的母牛受胎率比对照组高3.0%。
