Gluckman J C
Eur J Immunol. 1980 Sep;10(9):693-7. doi: 10.1002/eji.1830100907.
The mixed lymphocyte culture (MLC) is known to react to major histocompatibility complex disparities but is insensitive to minor histocompatibility antigens. To determine which modifications could amplify reactivity to these antigens, we have studied the effects of presensitization on normally nonresponsive MLC in dogs. Lymphocytes, primed in vitro to and restimulated with cells of a DLA-identical sib, did not react to these specific cells, in contrast to normal proliferation to other stimulators. However, lymphocytes obtained after in vivo sensitization against a DLA-identical donor showed increased reactivity to the donor in approximately half of the recipients. In comparison to primary MLC responses between DLA-mismatched individuals, the [3H] thymidine uptake was less important in this reaction, and peak incorporation was usually not accelerated. This response appeared to be specific in that responsiveness to third-party cells was not increased, and, alternatively, immunization against a DLA haplo-identical donor did not modify reactivity to DLA-identical cells. This increased reactivity after alloimmunization may reflect differences in minor histocompatibility antigens although the skin graft survival time was not significantly shorter in MLC responders than in nonresponder recipients. It seems that in vivo priming is required to expand the number of cells specific for these antigens and to enable detection of proliferation in MLC.
已知混合淋巴细胞培养（MLC）对主要组织相容性复合体差异有反应，但对次要组织相容性抗原不敏感。为了确定哪些修饰可以增强对这些抗原的反应性，我们研究了预致敏对犬正常无反应性MLC的影响。与对其他刺激物的正常增殖相反，体外致敏并再次用DLA相同的同胞细胞刺激的淋巴细胞对这些特定细胞无反应。然而，在体内对DLA相同供体致敏后获得的淋巴细胞在大约一半的受者中对供体的反应性增加。与DLA不匹配个体之间的初次MLC反应相比，该反应中[3H]胸苷摄取不太重要，并且峰值掺入通常没有加速。这种反应似乎具有特异性，因为对第三方细胞的反应性没有增加，并且，相反，对DLA单倍体相同供体的免疫并未改变对DLA相同细胞的反应性。尽管MLC反应者的皮肤移植存活时间并不比无反应受者明显短，但同种免疫后这种增加的反应性可能反映了次要组织相容性抗原的差异。似乎需要体内致敏来扩大针对这些抗原的特异性细胞数量，并能够在MLC中检测到增殖。
