Iseki S, Ebina T, Ishida N
Gan. 1980 Aug;71(4):567-71.
Caffeine was found to act as a stimulator of the recovery of HeLa-S3 cells from the G2 phase arrest induced by a cancer chemotherapeutic agent, neocarzinostatin (NCS). When the cells were incubated in the presence of caffeine after removal of NCS, the time required for the recovery of mitosis was dependent on the concentration of NCS and was markedly shortened by caffeine treatment in a concentration-dependent manner. When puromycin, a specific protein synthesis inhibitor, was added together with caffeine, a temporary recovery of mitosis was observed, followed by an irreversible arrest of mitosis, suggesting that the terminal point of action of NCS resides closer to the M phase than that of puromycin. These results indicate that the mechanism of NCS-induced G2 block is closely related to that of X-ray-induced G2 block.
研究发现，咖啡因可作为一种刺激物，促使HeLa-S3细胞从由癌症化疗药物新制癌菌素（NCS）诱导的G2期阻滞中恢复。在去除NCS后，若细胞在咖啡因存在的情况下进行孵育，恢复有丝分裂所需的时间取决于NCS的浓度，并且咖啡因处理能以浓度依赖的方式显著缩短该时间。当将特异性蛋白质合成抑制剂嘌呤霉素与咖啡因一起添加时，观察到有丝分裂出现暂时恢复，随后是有丝分裂的不可逆阻滞，这表明NCS的作用终点比嘌呤霉素更接近M期。这些结果表明，NCS诱导G2期阻滞的机制与X射线诱导G2期阻滞的机制密切相关。
