Tyler J P, Simpson J, Collins W P
J Reprod Fertil. 1980 Nov;60(2):295-300. doi: 10.1530/jrf.0.0600295.
The androgenic status of female mice was assessed by measuring the concentration of testosterone-17 beta-glucuronide in serial samples of unextracted urine. The subcutaneous administration of testosterone (50 micrograms in lauric acid ethyl ester) resulted in a significant increase (P < 0.01) of the mean +/- s.d. concentration of urinary testosterone-17 beta-glucuronide (25.9 +/- 6.5 to 71.7 +/- 12.9 ng/ml) within 2 h. The 2-h values after the administration of 50 micrograms androstenedione or 50 micrograms dehydroepiandrosterone were 22.8 +/- 4.4 to 81.8 +/- 7.8 ng/ml (P < 0.001) and 23.4 +/- 2.7 to 121.8 +/- 20.3 ng/ml (P < 0.001) respectively. The values decreased progressively over the next 24 h. After the induction of superovulation with PMSG and hCG, the values for testosterone-17 beta-glucuronide increased significantly (P < 0.01) during the periovulatory period (5--24 h after hCG injection). The mean +/- s.d. value in pregnancy was higher (61.8 +/- 11.6 ng/ml; P < 0.001) than that in non-pregnant animals (30.7 +/- 12.3 ng/ml) and remained relatively constant between Days 1 and 16.
通过测量未提取尿液系列样本中睾酮 - 17β - 葡萄糖醛酸苷的浓度来评估雌性小鼠的雄激素状态。皮下注射睾酮（50微克溶于月桂酸乙酯）导致尿液中睾酮 - 17β - 葡萄糖醛酸苷的平均±标准差浓度在2小时内显著增加（P < 0.01）（从25.9±6.5纳克/毫升增至71.7±12.9纳克/毫升）。注射50微克雄烯二酮或50微克脱氢表雄酮后2小时的值分别为22.8±4.4至81.8±7.8纳克/毫升（P < 0.001）和23.4±2.7至121.8±20.3纳克/毫升（P < 0.001）。在接下来的24小时内这些值逐渐下降。用孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）诱导超排卵后，在排卵前期（hCG注射后5 - 24小时）睾酮 - 17β - 葡萄糖醛酸苷的值显著增加（P < 0.01）。怀孕小鼠的平均±标准差的值（61.8±11.6纳克/毫升；P < 0.001）高于未怀孕动物（30.7±12.3纳克/毫升），并且在第1天至第16天之间保持相对恒定。
