Tze W J, Tai J, Wong F C
Metabolism. 1980 Oct;29(11):1020-5. doi: 10.1016/0026-0495(80)90210-3.
Pancreatic islets from adult rats, accumulated daily for a period of 2 wk, were cultured in artificial capillary culture units that were perfused with nutrient medium. Viability and functional capacity of these islets in this in vitro culture system has been shown to be maintained. The therapeutic efficiency of cultured islets was comparable to freshly isolated islets when assessed by transplantation into streptozotocin-induced diabetic rats. Intraportal implantation of either freshly isolated or cultured islets into isogeneic diabetic rats resulted in normal weight gain as well as complete reversal of hyperglycemia, glycosuria, and polyuria in the diabetic rat recipient. The normoglycemic state was sustained for more than 12 mo. Plasma glucose clearance rate (KG) were significantly lower in both the transplanted fresh or cultured islet groups than in the normal controls; however, the cultured islets were not less effective than the fresh islets. The results of the present study indicate the feasibility of accumulation of large numbers of islets in the in vitro culture system without any loss of therapeutic efficiency when implanted in vivo. Further improvement of this in vitro culture system can thus be applied in islet preservation for islet transplantation.
成年大鼠的胰岛，连续2周每天收集，在灌注营养培养基的人工毛细管培养装置中培养。这些胰岛在该体外培养系统中的活力和功能能力已被证明得以维持。当通过移植到链脲佐菌素诱导的糖尿病大鼠中进行评估时，培养的胰岛的治疗效果与新鲜分离的胰岛相当。将新鲜分离或培养的胰岛门静脉内植入同基因糖尿病大鼠，导致糖尿病大鼠受体体重正常增加，高血糖、糖尿和多尿完全逆转。正常血糖状态持续超过12个月。移植新鲜或培养胰岛组的血浆葡萄糖清除率（KG）均显著低于正常对照组；然而，培养的胰岛并不比新鲜胰岛效果差。本研究结果表明，在体外培养系统中积累大量胰岛而在体内植入时不损失任何治疗效率是可行的。因此，该体外培养系统的进一步改进可应用于胰岛移植的胰岛保存。
