Cerilli J, Newhouse Y G, Fesperman D P, Brasile L
Surgery. 1980 Nov;88(5):631-5.
Eighty-one donor-recipient pairs were evaluated prior to renal transplantation to obtain histocompatibility profiles. Standard tissue typing was used to detect serologically defined A and B locus antigens, and mixed lymphocyte cultures were employed to detect lymphocyte defined antigens. Results of both tests were correlated with each other and with allograft rejection. It was shown that as serologically defined histocompatibility at the A and B loci decreases, both the rate of graft rejection and the percentage of high mixed lymphocyte culture stimulation increase. Within each serologically defined category were found patients with a high and a low stimulation index in mixed lymphocyte culture. Regardless of the degree of serologically defined histocompatibility, patients with a high stimulation index had a statistically significant higher graft rejection rate than did patients with a low stimulation index. It appears that the mixed lymphocyte culture assay is a method superior to standard tissue typing in predicting renal allograft rejection with related donors, and therefore all potential donors for renal transplantation should be screened, utilizing the mixed lymphocyte culture technique.
在肾移植前对81对供受者进行了评估，以获得组织相容性图谱。采用标准组织分型来检测血清学定义的A和B位点抗原，并采用混合淋巴细胞培养来检测淋巴细胞定义的抗原。两种检测结果相互关联，并与同种异体移植排斥反应相关。结果表明，随着血清学定义的A和B位点组织相容性降低，移植排斥率和高混合淋巴细胞培养刺激百分比均增加。在每个血清学定义的类别中，都发现了混合淋巴细胞培养刺激指数高和低的患者。无论血清学定义的组织相容性程度如何，刺激指数高的患者移植排斥率在统计学上显著高于刺激指数低的患者。看来，在预测亲属供者肾移植排斥反应方面，混合淋巴细胞培养试验是一种优于标准组织分型的方法，因此，应利用混合淋巴细胞培养技术对所有潜在的肾移植供者进行筛查。
