Perrins D J, Wiernik G, Jones W A
Acta Haematol. 1980;64(2):72-8. doi: 10.1159/000207214.
An in vivo system has been developed for estimating the inhibitory activities of granulocyte chalone which overcomes the objection to the use of mixed proliferating target cells. It depends on the identification of labelled mature granulocytes in the peripheral blood of mice that received a radioactive precursor of DNA synthesis, with and without granulocyte extracts, several days previously. Two extracts of bovine granulocytes were tested. The first demonstrated that maximum inhibition was achived when it was given 2 h before the isotope, suggesting that its activity was short lived. It also failed to depress the labelling index of peripheral lymphocytes, thereby demonstrating tissue specificity. The second was used to show that the dose-response relationship was exponential.
已开发出一种体内系统，用于评估粒细胞抑素的抑制活性，该系统克服了使用混合增殖靶细胞的缺陷。它依赖于对小鼠外周血中标记成熟粒细胞的鉴定，这些小鼠在数天前分别接受了DNA合成放射性前体，有的还同时接受了粒细胞提取物。对两种牛粒细胞提取物进行了测试。第一种提取物表明，在同位素注射前2小时给予时可实现最大抑制，这表明其活性持续时间较短。它也未能降低外周淋巴细胞的标记指数，从而证明了组织特异性。第二种提取物用于表明剂量反应关系呈指数关系。
