一种筛选大肠杆菌K-12限制和修饰缺陷型突变体的简便方法。
An easy method for the selection of restriction- and modification-deficient mutants of Escherichia coli K-12.
作者信息
Piechaczyk M, Jeanteur P, Louarn J M
出版信息
Gene. 1980 Oct;11(1-2):173-5. doi: 10.1016/0378-1119(80)90098-0.
PMID:6449403
Abstract
An easy and rapid method for selecting restriction- and modification-defective mutants of Escherichia coli K-12 is described. This method employs selection of tetracycline resistant lysogens after infection with lambda::Tn10 phage and results in a high yield of spontaneous rk-mk- and rk-mk+ mutants.
摘要
描述了一种简单快速的方法来筛选大肠杆菌K-12的限制和修饰缺陷型突变体。该方法利用λ::Tn10噬菌体感染后筛选四环素抗性溶原菌，可高产自发的rk-mk-和rk-mk+突变体。
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