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从大肠杆菌K-12中形成λ（Tn10）tyrR⁺特异性转导噬菌体。

Formation of a lambda (Tn10) tyrR+ specialized transducing bacteriophage from Escherichia coli K-12.

作者信息

Cobbett C S, Pittard J

出版信息

J Bacteriol. 1980 Dec;144(3):877-83. doi: 10.1128/jb.144.3.877-883.1980.

DOI:10.1128/jb.144.3.877-883.1980
PMID:6254949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC294748/
Abstract

The transposon Tn10, coding for resistance to tetracycline, was inserted close to the tyrR+ gene at min 28 on the Escherichia coli chromosome. The homology between this transposon and a lambda (Tn10) phage was employed to direct integration of lambda close to tyrR+ with subsequent isolation of a lambda (Tn10) tyrR+ transducing phage. Results of restriction endonuclease analysis of the transducing phage are presented.

摘要

编码对四环素耐药性的转座子Tn10插入到大肠杆菌染色体上28分钟处的tyrR⁺基因附近。利用该转座子与λ（Tn10）噬菌体之间的同源性，将λ噬菌体定向整合到tyrR⁺基因附近，随后分离出λ（Tn10）tyrR⁺转导噬菌体。本文给出了转导噬菌体的限制性内切酶分析结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bae/294748/4bc5ba1ec5ea/jbacter00573-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bae/294748/4bc5ba1ec5ea/jbacter00573-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4bae/294748/4bc5ba1ec5ea/jbacter00573-0023-a.jpg

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本文引用的文献

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[The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].[大肠杆菌中β-半乳糖苷酶（乳糖酶）的生物合成；诱导的特异性]
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2
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Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Endonuclease fingerprinting of plasmids mediating gentamicin resistance in an outbreak of hospital infections.医院感染暴发中介导庆大霉素耐药的质粒的核酸内切酶指纹图谱分析
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4
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J Bacteriol. 1982 Dec;152(3):1276-9. doi: 10.1128/jb.152.3.1276-1279.1982.
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6
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Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
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