Raess B U, Vincenzi F F
J Pharmacol Methods. 1980 Nov;4(3):273-83. doi: 10.1016/0160-5402(80)90019-4.
A simple semi-automated method for the determination of erythrocyte plasma membrane (Mg2+)-ATPase, (Na+ + K+ + Mg2+)-ATPase, and the (Ca2+ + Mg2+)-ATPase is described in detail. A nonautomated ATPase enzyme assay for multiple membrane ATPases and automated methods for determination of inorganic phosphate as well as membrane-bound protein are described. Together, the methods represent a system that has the flexibility and sensitivity for basic research applications and the capacity to process relatively large numbers of samples as is common in clinical laboratory screening.
详细描述了一种用于测定红细胞质膜（Mg2 +）-ATP酶、（Na + + K + + Mg2 +）-ATP酶和（Ca2 + + Mg2 +）-ATP酶的简单半自动方法。还描述了一种用于多种膜ATP酶的非自动化ATP酶测定方法以及用于测定无机磷酸盐和膜结合蛋白的自动化方法。这些方法共同构成了一个系统，该系统具有基础研究应用所需的灵活性和灵敏度，并且具备处理相对大量样本的能力，这在临床实验室筛查中很常见。
