Kunori T, Ringdén O
Tissue Antigens. 1980 May;15(5):483-91. doi: 10.1111/j.1399-0039.1980.tb00212.x.
Antibody secreting B cells were measured as plaque forming cells (PFC) in a hemolysis-in-gel assay using fluorescein isothiocyanate (FITC) coupled SRBC as targets or protein A coupled SRBC as targets and developing antisera. Peak antibody secretion occurred on day 5 and the highest number of PFC was seen when mitomycin treated stimulator cells: responder cells were used in a ratio of 1:4. The number of PFC in MLC was not correlated to the DNA synthetic response. Antibody secretion in MLC was found to be of IgM, IgG and IgA classes. Significant numbers of PFC in MLC from blood lymphocytes were detected with the protein A technique, but not using FITC-SRBC targets. Compared to spleen cells, fewer PFC were stimulated in blood lymphocytes. B cells alone, enriched by rosetting of T cells, did not respond by antibody secretion or DNA synthesis in MLC. When Lipopolysaccharide (LPS) was added to MLC an additional effect was seen on the number of PFC which may indicate that distinct B cell subpopulations are activated in MLC by LPS.
在凝胶溶血试验中，以异硫氰酸荧光素（FITC）偶联的绵羊红细胞（SRBC）或蛋白A偶联的SRBC为靶细胞，并使用抗血清，将分泌抗体的B细胞测定为噬斑形成细胞（PFC）。抗体分泌高峰出现在第5天，当丝裂霉素处理的刺激细胞与反应细胞以1:4的比例使用时，可见到最高数量的PFC。混合淋巴细胞培养（MLC）中PFC的数量与DNA合成反应无关。发现MLC中的抗体分泌为IgM、IgG和IgA类。用蛋白A技术在血液淋巴细胞的MLC中检测到大量PFC，但使用FITC-SRBC靶细胞时未检测到。与脾细胞相比，血液淋巴细胞中被刺激产生的PFC较少。单独通过T细胞玫瑰花结富集的B细胞在MLC中不通过抗体分泌或DNA合成做出反应。当向MLC中添加脂多糖（LPS）时，可观察到对PFC数量的额外影响，这可能表明LPS在MLC中激活了不同的B细胞亚群。
