Kunori T, Ringdén O
Scand J Immunol. 1979;10(5):487-91. doi: 10.1111/j.1365-3083.1979.tb01379.x.
In mixed leucocyte culture (MLC), using human spleen cells or thoracic duct lymphocytes, antibody secretion was induced, measured as plaque-forming cells (PFC) in a haemolysis-in-gel assay with fluorescein isothiocyanate (FITC)-coupled sheep erythrocytes (SRBC) as targets. Peak antibody secretion was seen on day 5. Using protein-A-coupled SRBC as targets and developing antisera, antibody secretion in MLC was found to be of IgM, IgG and IgA type. There was no correlation between the number of PFC against FITC-SRBC in MLC and DNA synthesis. Supernatants from MLC failed to induce antibody secretion.
在混合淋巴细胞培养（MLC）中，使用人脾细胞或胸导管淋巴细胞诱导抗体分泌，通过凝胶溶血试验以异硫氰酸荧光素（FITC）偶联的绵羊红细胞（SRBC）作为靶标，将抗体分泌量测定为噬斑形成细胞（PFC）。在第5天观察到抗体分泌高峰。以蛋白A偶联的SRBC作为靶标并制备抗血清，发现MLC中的抗体分泌为IgM、IgG和IgA类型。MLC中针对FITC-SRBC的PFC数量与DNA合成之间没有相关性。MLC的上清液未能诱导抗体分泌。
