Riviere G R, Tarbox G S
Transplantation. 1980 Oct;30(4):290-3. doi: 10.1097/00007890-198010000-00010.
Twenty-six rhesus monkeys were paired on the basis of RhLA serologically defined (SD) antigens and then mixed lymphocyte cultures (MLCs) were performed for each donor-recipient combination. All but one monkey responded positively to its partner in MLC. In vitro assays for complement-dependent antibody-mediated cytotoxicity (CDC) and lymphocyte-mediated cytotoxicity (LMC) were performed before and after each of two separate orthotopic tooth transplants for each recipient. There was no detectable CDC or LMC before tooth transplantation. The maxillary right central incisors were exchanged between paired monkeys and 60 days later the left maxillary central incisors were transplanted. After the first tooth allograft, there was very little CDC activity in any recipient and only one matched group showed any remarkable LMC activity. However, after the second tooth transplant there was a very clear CDC response in the majority of monkeys. The LMC activity remained low after retransplantation. There did not seem to be any consistent relationship between RhLA match and in vitro cytotoxicity. The results indicate that tooth allografts are weak immunogens but they do evoke humoral immunity after retransplantation. The biological relevance of these serum antibodies remains to be determined.
26只恒河猴根据RhLA血清学定义（SD）抗原进行配对，然后对每对供体-受体组合进行混合淋巴细胞培养（MLC）。除一只猴子外，所有猴子在MLC中对其配对伙伴均产生阳性反应。对每个受体进行两次单独的原位牙移植，每次移植前后均进行补体依赖抗体介导的细胞毒性（CDC）和淋巴细胞介导的细胞毒性（LMC）的体外检测。牙移植前未检测到CDC或LMC。在上颌右中切牙在配对猴子之间进行交换，60天后进行左上颌中切牙移植。第一次牙移植后，任何受体的CDC活性都很低，只有一组匹配组显示出明显的LMC活性。然而，第二次牙移植后，大多数猴子出现了非常明显的CDC反应。再次移植后LMC活性仍然很低。RhLA匹配与体外细胞毒性之间似乎没有任何一致的关系。结果表明，牙移植是弱免疫原，但在再次移植后确实会引发体液免疫。这些血清抗体的生物学相关性仍有待确定。
