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[大肠杆菌K-12 λ噬菌体受体在转染和质粒转化中的作用]

[Role of Escherichia coli K-12 lambda phage receptors in transfection and plasmid transformation].

作者信息

Likhacheva N A, Molchanova E S, Zakharenko V I, Il'iashenko B N

出版信息

Genetika. 1980;16(7):1176-81.

PMID:6450083
Abstract

The dependence of competence of Escherichia coli cells in transfection and plasmid transformation from phage lambda receptor protein was studied. Mal+ variants, sensitive to phage lambda (Mal+ lambda S) were obtained from strains with impaired lambda-phage receptor protein (Mal-lambda R). Maximum increase of transfection (4.7-fold) was observed in JC411Mal+ lambda S strain. The efficiency of plasmid transformation of pMB9 and RP4 DNAs was also higher in the strain with functioning lambda-receptor protein.

摘要

研究了大肠杆菌细胞在转染和质粒转化中对噬菌体λ受体蛋白的依赖性。从λ噬菌体受体蛋白受损的菌株(Mal-λR)中获得了对噬菌体λ敏感的Mal+变体(Mal+λS)。在JC411Mal+λS菌株中观察到转染的最大增加(4.7倍)。在具有功能性λ受体蛋白的菌株中,pMB9和RP4 DNA的质粒转化效率也更高。

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