Attarian D E, Jones R N, Currie W D, Hill R C, Sink J D, Olsen C O, Chitwood W R, Wechsler A S
J Thorac Cardiovasc Surg. 1981 Mar;81(3):382-8.
Using a canine model of subcoronary valvular aortic stenosis, we determined myocardial blood flow, high-energy phosphate content, and mitochondrial function in eight hearts with chronic left ventricular hypertrophy. Fourteen normal hearts were used for control data. Myocardial blood flow was determined by injection of tracer microspheres. During cardiopulmonary bypass, left ventricular transmural biopsy specimens were taken for metabolic analyses. Subepicardial and subendocardial content of adenosine triphosphate (ATP) and creatine phosphate (CP) were assayed. Respiratory control indices for isolated mitochondria were measured by use of NAD-linked and FAD-linked substrates. Endocardial blood flow, subendocardial high-energy phosphate content, and respiratory control indices for NAD-linked substrate in the hearts with chronic left ventricular hypertrophy were significantly lower than the normal values. These data provide insight into the metabolic and myocardial blood flow abnormalities occurring in cardiac hypertrophy and provide a framework for understanding the altered response of hypertrophied hearts to ischemia.
利用冠状动脉下瓣膜性主动脉狭窄的犬模型，我们测定了8只患有慢性左心室肥厚的心脏的心肌血流量、高能磷酸盐含量和线粒体功能。14只正常心脏用作对照数据。通过注射示踪微球来测定心肌血流量。在体外循环期间，获取左心室透壁活检标本进行代谢分析。测定了三磷酸腺苷（ATP）和磷酸肌酸（CP）的心外膜下和心内膜下含量。通过使用与NAD相关和与FAD相关的底物来测量分离线粒体的呼吸控制指数。慢性左心室肥厚心脏的心内膜血流量、心内膜下高能磷酸盐含量以及与NAD相关底物的呼吸控制指数显著低于正常值。这些数据为了解心脏肥大中发生的代谢和心肌血流异常提供了见解，并为理解肥厚心脏对缺血的改变反应提供了框架。
