Hokland P, Heron I
Scand J Immunol. 1980;12(3):183-91. doi: 10.1111/j.1365-3083.1980.tb00058.x.
Two human T lymphocyte subpopulations were purified on the basis of their different affinities to sheep erythrocytes and compared with unseparated and non-T lymphocytes as effectors in cell-mediated lympholysis (CML) after mixed lymphocyte culture (MCL), in antibody-dependent cellular cytotoxicity (ADCC) against the mouse mastocytoma line P 815, and in spontaneous cell-mediated cytotoxicity (SCMC) against the human myeloid leukaemia line K 562 and the Burkitt lymphoma line RAJI. The low-avidity T cells (T1) had developed into more potent effectors than the high-avidity (Te) when assessed in CML. In ADCC and SCMC both subsets exhibited low but consistent lysis with no demonstrable differences. Addition of human leucocyte interferon to the ADCC and SCMC cultures resulted in enhanced SCMC, most notable and to an equal extent with the T cell subsets. In contrast, ADCC of unseparated cells, non-T cells, and the two T subsets was found to be unchanged after interferon addition. These data are discussed in relation to findings with human T-cell subpopulations purified by other methods.
根据对绵羊红细胞亲和力的不同，纯化出两个人类T淋巴细胞亚群，并将其与未分离的细胞及非T淋巴细胞进行比较，作为混合淋巴细胞培养（MCL）后细胞介导的淋巴细胞溶解（CML）、针对小鼠肥大细胞瘤系P 815的抗体依赖性细胞毒性（ADCC）以及针对人类髓系白血病系K 562和伯基特淋巴瘤系RAJI的自发细胞介导细胞毒性（SCMC）中的效应细胞。在CML中评估时，低亲和力T细胞（T1）已发展成为比高亲和力T细胞（Te）更有效的效应细胞。在ADCC和SCMC中，两个亚群均表现出低但一致的细胞溶解，没有明显差异。向ADCC和SCMC培养物中添加人白细胞干扰素导致SCMC增强，最显著且在两个T细胞亚群中程度相同。相比之下，发现添加干扰素后，未分离细胞、非T细胞和两个T亚群的ADCC没有变化。结合用其他方法纯化的人类T细胞亚群的研究结果对这些数据进行了讨论。
