Bures L, Heller J, Janácek K, Rybová R, Kleinová M, Kolc J, Málek P
Physiol Bohemoslov. 1980;29(6):569-75.
The authors studied the effect of Mercurascan (MSC) (a hydroxy- mercury derivative of fluorescein) on electrical parameters, namely potential difference (P.D.) and short circuit current (S.C.C.) of frog skin and on the ability of frog bladder tissue to accumulate sodium ions in experiments in vitro. It was found that MSC, in 10(-4) mol/l concentration, reduced the S.C.C., after a brief initial increase, to 5% of the original value and that the P.D. fell steadily right from the outset. In 10(-5) mol/l concentration it raised the S.C.C. by 60% and the increase lasted several hours. The P.D. was unaffected. In 10(-7) and 10(-6) mol/l concentration MSC had no effect on the NA+ content of a nonpolarized frog bladder tissue preparation, but a 10(-5) nol/l concentration sharply reduced it. The effect of MSC on membrane Na+--K+ ATPase, i.e. on the energy metabolism of cellular tissue, is discussed with reference to these results.
作者在体外实验中研究了汞荧光素扫描剂（MSC）（一种荧光素的羟基汞衍生物）对青蛙皮肤电参数，即电位差（P.D.）和短路电流（S.C.C.）的影响，以及对青蛙膀胱组织积累钠离子能力的影响。研究发现，浓度为10⁻⁴mol/L的MSC在最初短暂升高后，将S.C.C.降低至原始值的5%，并且P.D.从一开始就持续下降。浓度为10⁻⁵mol/L时，它使S.C.C.提高了60%，且这种增加持续了几个小时。P.D.未受影响。浓度为10⁻⁷和10⁻⁶mol/L时，MSC对未极化的青蛙膀胱组织制剂的钠离子含量没有影响，但浓度为10⁻⁵mol/L时会使其急剧降低。参照这些结果，讨论了MSC对膜钠钾ATP酶，即对细胞组织能量代谢的影响。
