Stegmayr B, Gottfries A, Ronquist G, Brody I
Scand J Urol Nephrol. 1980;14(2):129-34. doi: 10.3109/00365598009179549.
Mg2+- and Ca2+-dependent ATPase activity, fructose and divalent cation concentrations were determined in seminal plasmas from men with oligozoospermic ejaculates and from those with normal sperm count. The mean activity of the Mg2+- and Ca2+-stimulated ATPase from the former was 59 nmol . 0.1 ml-1 . This is significantly (p less than 0.005) lower than for men with a normal sperm count (corresponding figure is 87 nmol . 0.1m1-1 . min-1). As regards the concentrations of Mg2+, Ca2+ and Zn2+ in the seminal plasmas from these two groups there was no significant difference. The same was also valid for the fructose concentrations. The Mg2+- and Ca2+-stimulated ATPase activity as well as fructose and divalent cation concentrations were also determined in seminal plasmas from men before and after vasectomy. After vasectomy, only the ejaculates devoid of spermatozoa were included in the comparative studies. No significant difference was observed between the seminal plasmas from men before and after vasectomy. These findings support the view that the Mg2+- and Ca2+-stimulated ATPase system does not derive from the spermatozoa. Possible explanations for the significantly lowered Mg2+- and Ca2+-dependent ATPase activity from patients with oligozoospermia are given.
测定了少精子症患者和精子计数正常者精液中的镁离子和钙离子依赖性ATP酶活性、果糖及二价阳离子浓度。少精子症患者精液中镁离子和钙离子刺激的ATP酶平均活性为59 nmol·0.1 ml⁻¹·min⁻¹，显著低于精子计数正常者（相应数值为87 nmol·0.1m1⁻¹·min⁻¹，p<0.005）。两组精液中镁离子、钙离子和锌离子的浓度无显著差异，果糖浓度也是如此。还测定了输精管结扎术前、后男性精液中的镁离子和钙离子刺激的ATP酶活性以及果糖和二价阳离子浓度。输精管结扎术后，比较研究仅纳入无精子的精液。输精管结扎术前、后男性的精液无显著差异。这些发现支持镁离子和钙离子刺激的ATP酶系统并非来源于精子的观点。文中给出了少精子症患者镁离子和钙离子依赖性ATP酶活性显著降低的可能解释。
