Stadnicka A
Acta Histochem. 1980;67(2):227-33. doi: 10.1016/S0065-1281(80)80026-2.
The Timm sulphide-silver method was used for histochemical localization of mercury in the ovaries of young rats fed with mercuric chloride. After 12 weeks of mercuric chloride administration test animals showed disturbances in the estrous cycle manifested by the prolongation of diestrous phase. In the ovaries of these rats the deposits of mercury were shown to occur particularly in macrophages of atretic young and mature ovarian follicles and corpora lutea, within granulosa cells of atretic follicles, and in the lutein cells of freshly formed corpora lutea. Follicular fluid and, irregularly, germinal epithelium also showed the ability to mercury accumulation. The ovaries of control animals treated according to the Timm procedure demonstrated negative histochemical reactivity.
采用Timm硫化银法对喂食氯化汞的幼鼠卵巢中的汞进行组织化学定位。在给予氯化汞12周后，试验动物的发情周期出现紊乱，表现为动情间期延长。在这些大鼠的卵巢中，汞沉积尤其出现在闭锁的幼年和成熟卵泡及黄体的巨噬细胞中、闭锁卵泡的颗粒细胞内以及新形成黄体的黄体细胞中。卵泡液以及偶尔的生发上皮也显示出积累汞的能力。按照Timm程序处理的对照动物卵巢显示出阴性组织化学反应。
