Amirov E Ia
Zh Mikrobiol Epidemiol Immunobiol. 1980 Dec(12):21-4.
The formation of specifically transducing lambda dg-particles in E.coli strain W3110 (lambda) due to the effect of different doses of UV irradiation was studied. A sharp increase in both the absolute number of transductants and the relative transduction frequency was found to occur as the dose of UV irradiation increased. On achieving definite doses this relationship became almost direct. At higher doses similarity was observed in the kinetics of a decrease in the absolute number of transductants and in active phage titers. As the dose increased further, a sharp increase in the percentage of heterogenoue transductants occurred. The conclusion that the formation of specifically transducing lambda dg-particles is due to the effect of the inducing factor on the DNA of lysogenic cells has been made.
研究了不同剂量紫外线照射对大肠杆菌菌株W3110（λ）中特异性转导λdg颗粒形成的影响。发现随着紫外线照射剂量的增加，转导子的绝对数量和相对转导频率均急剧增加。当达到一定剂量时，这种关系几乎呈线性。在较高剂量下，观察到转导子绝对数量和活性噬菌体滴度下降的动力学相似。随着剂量进一步增加，异源转导子的百分比急剧增加。得出结论：特异性转导λdg颗粒的形成是由于诱导因子对溶原细胞DNA的作用。
