Porter R D, Welliver R A, Witkowski T A
J Bacteriol. 1982 Jun;150(3):1485-8. doi: 10.1128/jb.150.3.1485-1488.1982.
Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution transductants. When a chromosomal lac gene was the recipient DNA substrate molecule, both the normalized transduction frequency and the proportion of addition and substitution transductants showed essentially no recB dependence. There was a pronounced recB dependence for both normalized transduction frequency and recombination end product formation when F42 lac was the recipient DNA substrate. recB appears to have no significant role in the recombination that occurs between the two lac regions in an addition transductant. UV irradiation of the transducing phages increased the absolute level of both addition and substitution transductants obtained with a chromosomal lac gene but resulted in a considerable change in the relative frequency of addition versus substitution transductants.
利用基因缺陷型λplac5转导噬菌体衍生物来研究特异性转导过程中重组对recB的依赖性。将转导频率标准化为菌落形成单位，并通过对添加型和替代型转导子进行评分来监测重组的终产物。当染色体lac基因作为受体DNA底物分子时，标准化转导频率以及添加型和替代型转导子的比例基本上都不依赖recB。当F42 lac作为受体DNA底物时，标准化转导频率和重组终产物形成均明显依赖recB。recB似乎在添加型转导子中两个lac区域之间发生的重组中没有显著作用。对转导噬菌体进行紫外线照射增加了用染色体lac基因获得的添加型和替代型转导子的绝对水平，但导致添加型与替代型转导子的相对频率发生了相当大的变化。
