Kuz'mishcheva E B, Krivopishin I P, Iurovitskiĭ Iu G, Mil'man L S
Ontogenez. 1981;12(3):266-72.
In the skeletal muscles of the chick embryo from the 10th till the 15th day of embryogenesis, phosphorylase (EC. 2.4.1.1) is represented by two isozymes one of which corresponds, by electrophoretic mobility, to the liver phosphorylase and another to phosphorylase of the skeletal muscles of the adult rat. From the 17th day of embryogenesis on only one isozyme of phosphorylase is found in the skeletal muscles which is identical with that of the skeletal muscles of the adult bird. The isozyme spectrum of phosphorylase of the whole 4 days old embryo contains, besides phosphorylase L, a special "embryonic" isozyme which differs from that of the skeletal muscles by immunochemical characteristics and electrophoretic mobility. From the 10th day of embryogenesis till hatching, the activity of phosphorylase of the skeletal muscles increases more than 50 times and that of glycogen synthetase (EC. 2.4.1.11) only 4 times.
在胚胎发育第10天至15天的鸡胚骨骼肌中,磷酸化酶(EC. 2.4.1.1)由两种同工酶代表,其中一种通过电泳迁移率与肝脏磷酸化酶相对应,另一种与成年大鼠骨骼肌的磷酸化酶相对应。从胚胎发育第17天起,在骨骼肌中仅发现一种磷酸化酶同工酶,它与成年鸟类骨骼肌的同工酶相同。整个4日龄胚胎的磷酸化酶同工酶谱除了磷酸化酶L外,还含有一种特殊的“胚胎”同工酶,其免疫化学特性和电泳迁移率与骨骼肌的不同。从胚胎发育第10天到孵化,骨骼肌中磷酸化酶的活性增加了50多倍,而糖原合成酶(EC. 2.4.1.11)的活性仅增加了4倍。