Vaickus L, Tomasi T B
Transplantation. 1981 Jun;31(6):409-16. doi: 10.1097/00007890-198106000-00002.
In an attempt to determine whether culture conditions significantly influence autoreactivity, we tested the effects of fetal calf, syngeneic, and autogeneic serum with and without 2-mercaptoethanol on the murine self-reactive mixed lymphocyte culture. We used both unfractionated and T-enriched lymph node cells as responders and unfractionated and B-enriched irradiated spleen cells as stimulators in a primary mixed lymphocyte culture (MLC). Using unfractionated cells as responders to various stimulator cell populations, we found excellent allogeneic reactions in all media tested but minimal or no self-reactivity in syngeneic of autogeneic serum. When using T-enriched lymph node cells as responders, allogeneic reactivity was excellent but self-reactivity was present only in the cultures supplemented with fetal calf serum and 2-mercaptoethanol. The possibility that the substances in fetal calf serum and/or 2-mercaptoethanol may be needed to enhance minimal, but biologically relevant, self-reactivity is discussed, as well as the possibility that the culture supplements may be inducing "nonspecific" self-reactivity.
为了确定培养条件是否会显著影响自身反应性，我们测试了添加和不添加2-巯基乙醇的胎牛血清、同基因血清和自体血清对小鼠自身反应性混合淋巴细胞培养的影响。在初次混合淋巴细胞培养（MLC）中，我们使用未分离的和T细胞富集的淋巴结细胞作为反应细胞，以及未分离的和B细胞富集的经照射的脾细胞作为刺激细胞。使用未分离的细胞作为对各种刺激细胞群体的反应细胞，我们发现在所有测试培养基中同种异体反应良好，但在同基因或自体血清中自身反应性极小或无自身反应性。当使用T细胞富集的淋巴结细胞作为反应细胞时，同种异体反应性良好，但仅在添加胎牛血清和2-巯基乙醇的培养物中存在自身反应性。本文讨论了可能需要胎牛血清和/或2-巯基乙醇中的物质来增强极小但具有生物学相关性的自身反应性，以及培养补充剂可能诱导“非特异性”自身反应性的可能性。
