Pullen A M, Munro A J
Department of Pathology, University of Cambridge, U.K.
Immunology. 1988 Feb;63(2):255-60.
During attempts to generate Sendai virus-specific T-cell hybridomas, a number of autoreactive T-cell hybridomas were produced. These hybrids secrete IL-2 in response to class II-positive syngeneic cells in the absence of added Sendai virus. Class II molecules on the stimulator cells are involved since monoclonal anti-class II antibodies block the reaction, and mapping studies using recombinant mouse strains show the response is class II restricted. Hybridomas adapted to grow in serum-free medium do not respond to syngeneic cells in the absence of serum; however, addition of fetal calf serum (FCS) restores the response. The component of FCS responsible for the stimulation of the hybridomas has been partially purified and characterized, and its mode of action investigated. These findings suggest that the putative 'autoreactive' T-cell hybridomas reported by others may be specific for a component of the culture medium rather than being truly self-reactive.
在尝试生成仙台病毒特异性T细胞杂交瘤的过程中，产生了许多自身反应性T细胞杂交瘤。这些杂交瘤在未添加仙台病毒的情况下，对II类阳性同基因细胞产生反应并分泌白细胞介素-2。刺激细胞上的II类分子参与其中，因为单克隆抗II类抗体可阻断该反应，并且使用重组小鼠品系的定位研究表明该反应受II类限制。适应在无血清培养基中生长的杂交瘤在无血清时对同基因细胞无反应；然而，添加胎牛血清（FCS）可恢复反应。负责刺激杂交瘤的FCS成分已被部分纯化和表征，并对其作用方式进行了研究。这些发现表明，其他人报道的假定“自身反应性”T细胞杂交瘤可能对培养基的一种成分具有特异性，而不是真正的自身反应性。
