Ingenito G G, Calkins C E
J Immunol. 1981 Sep;127(3):1236-40.
Spleen cells of DBA/2 mice bearing subcutaneous implants of the syngeneic tumor L5178Y induce suppression of the in vitro antibody response of normal spleen cells to sheep erythrocytes (SRBC). Cells mediating suppression are detected in the spleens of tumor-bearing mice as early as 24 hr post-implantation but are no longer detected there 15 days post-implantation. These spleen cells are nylon wool nonadherent, sensitive to anti-Thy 1.2 + C and anti-Lyt 1.1 + C, and insensitive to anti-Lyt 2.1 + C treatment. The anti-SRBC response of the unfractionated spleen cells from the tumor-bearing mice is not itself suppressed at the cell numbers used. This along with the finding that suppression occurs in the presence of spleen cells from normal mice suggest that a cell population from the normal mouse spleen is also involved in the suppression. Spleen cells from mice inoculated with irradiated (nonproliferating) L5178Y cells are similarly capable of mediating nonspecific suppression for the same limited period of time after the inoculation. In addition, spleen cells from mice stimulated with several nontumorigenic cellular antigens interact with normal spleen cells to produce suppression. These findings suggest that suppression observed in vitro with spleen cells from these tumor-bearing mice may be the result of antigen-activated cells triggering normal immunoregulatory cells.
携带同基因肿瘤L5178Y皮下植入物的DBA/2小鼠的脾细胞可抑制正常脾细胞对绵羊红细胞（SRBC）的体外抗体反应。早在植入后24小时就在荷瘤小鼠的脾脏中检测到介导抑制作用的细胞，但在植入后15天就不再在那里检测到。这些脾细胞不黏附尼龙毛，对抗Thy 1.2+C和抗Lyt 1.1+C敏感，而对抗Lyt 2.1+C处理不敏感。在所用细胞数量下，荷瘤小鼠未分级脾细胞的抗SRBC反应本身并未受到抑制。这一点以及在存在正常小鼠脾细胞的情况下发生抑制作用的发现表明，正常小鼠脾细胞中的一个细胞群体也参与了抑制作用。接种了经辐照（不增殖）L5178Y细胞的小鼠的脾细胞在接种后的相同有限时间内同样能够介导非特异性抑制作用。此外，用几种非致瘤性细胞抗原刺激的小鼠的脾细胞与正常脾细胞相互作用产生抑制作用。这些发现表明，用这些荷瘤小鼠的脾细胞在体外观察到的抑制作用可能是抗原激活的细胞触发正常免疫调节细胞的结果。
