Yoshida H, Akeho M, Okamoto K, Yumoto T
Acta Pathol Jpn. 1981 May;31(3):399-412. doi: 10.1111/j.1440-1827.1981.tb01384.x.
Seven transplantable leukemias and lymphomas which occurred in NZB mice were characterized immunologically and cytologically as B-cell type of L66-, 2709-, 2769- find S77-lines, and T-cell type of TH17-, TH90- and S29-lines. These were also studied with enzyme histochemistry in tissue sections. All B-cell tumors revealed strong activities of ATPase and 5'-nucleotidase and one expressed A1Pase activity on the cell surface. Two thymic lymphomas (TH17- and TH90-lines) and one T-cell leukemia (S29-line) showed negative reactions of ATPase and 5'-nucleotidase but positive activities of AcPase and non-specific esterase localized in their cytoplasms. A1Pase activity was expressed in TH90-lymphoma but not in TH17- and S29-lymphomas. A1Pase recognized in L66- and TH90-lymphomas showed similar reaction to those of Nagao's isoenzyme. The splenic follicles of normal NZB mice reacted to ATPase and 5'-nucleotidase in B-cell area (primary follicles) and to AcPase and non-specific esterase in T-cell area (PALS) with the localized reaction. Data suggest that four enzymes of ATPase, 5'-nucleotidase, AcPase and non-specific esterase are useful for the differentiation of B- and T-cells and their malignant counterparts.
在NZB小鼠中发生的7种可移植性白血病和淋巴瘤，经免疫学和细胞学鉴定为B细胞类型的L66 -、2709 -、2769 -和S77系，以及T细胞类型的TH17 -、TH90 -和S29系。还对这些肿瘤进行了组织切片的酶组织化学研究。所有B细胞肿瘤均显示出ATP酶和5'-核苷酸酶的强活性，其中一种在细胞表面表达ATP酶活性。两种胸腺淋巴瘤（TH17 -和TH90系）和一种T细胞白血病（S29系）显示ATP酶和5'-核苷酸酶呈阴性反应，但酸性磷酸酶（AcPase）和非特异性酯酶在其细胞质中呈阳性活性。TH90淋巴瘤表达ATP酶活性，而TH17和S29淋巴瘤则不表达。在L66和TH90淋巴瘤中识别出的ATP酶与长冈同工酶的反应相似。正常NZB小鼠的脾滤泡在B细胞区域（初级滤泡）对ATP酶和5'-核苷酸酶有反应，在T细胞区域（动脉周围淋巴鞘，PALS）对酸性磷酸酶和非特异性酯酶有局部反应。数据表明，ATP酶、5'-核苷酸酶、酸性磷酸酶和非特异性酯酶这四种酶有助于区分B细胞和T细胞及其恶性对应物。
