源自早期和/或成熟胸腺细胞的两种小鼠T细胞淋巴瘤的细胞表面抗原表型和酶表达模式。

Cell surface antigen phenotypes and enzyme expression patterns of two murine T-cell lymphomas derived from early and/or mature thymus cells.

作者信息

Dullens H F, Hilgers J, Van Basten C D, De Weger R A, De Heer E, Den Otter W

出版信息

Leuk Res. 1982;6(3):425-8. doi: 10.1016/0145-2126(82)90108-4.

Abstract

A number of cell surface markers (T200, ThB, Thy1, Lyt1 and Lyt2 and a glycolipid) and enzymes (ATP-ase, acid phosphatase, beta-glucuronidase, 5'-nucleotidase, non-specific esterase, ANAE and chloroacetate esterase) were determined for two murine T-cell lymphomas: the DBA/2-strain-derived SL2 with a phenotype close to that of a mature thymocyte and the GRS-strain-derived GSRL13 with a phenotype of a more primitive thymocyte. While the pattern of expression of the enzymes was similar for SL2 and GRSL13 and as such indistinguishable from that of the majority of thymus cells, the pattern of cell surface antigen expression was clearly different. GRSL12 cells express the ThB antigen and a glycolipid antigen detectable with monoclonal antibody 30-H11, but not Lyt1 and Lyt2 antigens. SL2 cells, however, do not express ThB and the glycolipid antigen, but do express Lyt1 and Lyt2.

摘要

针对两种小鼠T细胞淋巴瘤，测定了若干细胞表面标志物（T200、ThB、Thy1、Lyt1和Lyt2以及一种糖脂）和酶（ATP酶、酸性磷酸酶、β-葡萄糖醛酸酶、5'-核苷酸酶、非特异性酯酶、ANAE和氯乙酸酯酶）：来源于DBA/2品系的SL2，其表型接近成熟胸腺细胞；来源于GRS品系的GSRL13，其表型为更原始的胸腺细胞。虽然SL2和GRSL13的酶表达模式相似，因此与大多数胸腺细胞的酶表达模式无法区分，但细胞表面抗原的表达模式明显不同。GRSL12细胞表达ThB抗原和可被单克隆抗体30-H11检测到的糖脂抗原，但不表达Lyt1和Lyt2抗原。然而，SL2细胞不表达ThB和糖脂抗原，但表达Lyt1和Lyt2。