来自母鸡输卵管的1SF单体核小体的表征以及此类核小体中第三种HMG14/17样蛋白的部分表征。

Goodwin G H, Wright C A, Johns E W

Nucleic Acids Res. 1981 Jun 25;9(12):2761-75. doi: 10.1093/nar/9.12.2761.

Nucleosomes released from oviduct nuclei during brief micrococcal nuclease digestions are enriched in transcribed sequences (bloom K.S. and Anderson, J.N. (1978) Cell, 15, 141-150). Such nucleosomes released into this 1Sf supernatant fraction are enriched in proteins HMG14, 17 and a third lower molecular weight protein which we show in this paper to be related to HMG14 and 17. This protein, which we call HMGY, runs as a doublet on polyacrylamide gels. A similar doublet is present in smaller quantities in chicken erythrocyte nuclei. Monomer nucleosomes in the 1SF supernatant have been separated by polyacrylamide gel electrophoresis into two main bands. The slower moving band contains the three HMG proteins HMG14, 17 and Y but lacks histone H1.

在短暂的微球菌核酸酶消化过程中从输卵管细胞核释放的核小体富含转录序列（布鲁姆·K.S.和安德森，J.N.（1978年）《细胞》，15卷，141 - 150页）。释放到这个1Sf上清液组分中的此类核小体富含蛋白质HMG14、17以及第三种分子量较低的蛋白质，我们在本文中表明该蛋白质与HMG14和17相关。这种我们称为HMGY的蛋白质在聚丙烯酰胺凝胶上呈双峰形式迁移。在鸡红细胞核中也存在少量类似的双峰。1SF上清液中的单体核小体已通过聚丙烯酰胺凝胶电泳分离成两条主要条带。迁移较慢的条带包含三种HMG蛋白HMG14、17和Y，但缺乏组蛋白H1。