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一类富含高迁移率族蛋白14和17以及活跃转录的珠蛋白基因的核小体的电泳分离。

Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes.

作者信息

Albanese I, Weintraub H

出版信息

Nucleic Acids Res. 1980 Jun 25;8(12):2787-805. doi: 10.1093/nar/8.12.2787.

DOI:10.1093/nar/8.12.2787
PMID:6448987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324121/
Abstract

Monomer nucleosomes from chick erythrocytes can be fractionated according to their electrophoretic mobility in (comparatively) high salt acrylamide gels. We show that the fractionation is based predominantly on differences in charge. The monomer heterogeneity persists even when the nucleosomes are trimmed down to 145 bp with Exo III or when H1 and H5 are removed. The slowest migrating monomers are associated with HMG 14 and 17; however, we do not believe that these proteins are entirely responsible for the altered mobility since the nucleosome heterogeneity persists even after removal of HMG 14 and 17. The DNA associated with the HMG 14 and 17 containing nucleosomes is shown to be enriched in actively transcribed globin sequences.

摘要

鸡红细胞中的单体核小体可以根据它们在（相对）高盐丙烯酰胺凝胶中的电泳迁移率进行分级分离。我们发现这种分级分离主要基于电荷差异。即使使用外切核酸酶III将核小体剪切成145 bp，或者去除H1和H5，单体的异质性仍然存在。迁移最慢的单体与HMG 14和17相关；然而，我们认为这些蛋白质并非导致迁移率改变的唯一原因，因为即使去除HMG 14和17后，核小体的异质性依然存在。与含有HMG 14和17的核小体相关的DNA在活跃转录的珠蛋白序列中富集。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/f9f677e0e9e0/nar00429-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/f29a0f476914/nar00429-0230-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/31463c91b0e7/nar00429-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/c2790024630a/nar00429-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/ee5458693d9b/nar00429-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/de907f2340da/nar00429-0236-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/309d8544062c/nar00429-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/7f66c60e6e42/nar00429-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/f9f677e0e9e0/nar00429-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/f29a0f476914/nar00429-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/e79c91d597af/nar00429-0230-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/81257bc2c290/nar00429-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/31463c91b0e7/nar00429-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/c2790024630a/nar00429-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/ee5458693d9b/nar00429-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/de907f2340da/nar00429-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/19a6ee55cb0a/nar00429-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/579c284a1bb7/nar00429-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/309d8544062c/nar00429-0240-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/7f66c60e6e42/nar00429-0241-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e49/324121/f9f677e0e9e0/nar00429-0243-a.jpg

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本文引用的文献

1
Interaction of HMG 14 and 17 with actively transcribed genes.HMG 14和17与活跃转录基因的相互作用。
Cell. 1980 Jan;19(1):289-301. doi: 10.1016/0092-8674(80)90410-9.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
体外HMG-I/Y依赖于SAR的组蛋白H1动员：HMG-I/Y在H1缺失的染色质中富集。
EMBO J. 1993 Aug;12(8):3237-47. doi: 10.1002/j.1460-2075.1993.tb05993.x.
4
Transferring DNA from electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose: properties of nucleosomes along mouse satellite DNA.将电泳分离的核小体中的DNA转移至重氮苄氧基甲基纤维素：小鼠卫星DNA上核小体的特性
Nucleic Acids Res. 1982 Feb 25;10(4):1311-25. doi: 10.1093/nar/10.4.1311.
5
Chromatin assembled in the presence of cytosine arabinoside has a short nucleosome repeat.在阿糖胞苷存在的情况下组装的染色质具有短的核小体重复序列。
Nucleic Acids Res. 1983 Aug 25;11(16):5451-66. doi: 10.1093/nar/11.16.5451.
6
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7
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Nucleic Acids Res. 1983 Feb 11;11(3):753-72. doi: 10.1093/nar/11.3.753.
8
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9
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5
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