Ketlinskiĭ S A, Svetikova K M
Arkh Anat Gistol Embriol. 1981 Jul;81(7):64-71.
Non-inbred and hybrid mice, line C57Bl and CBA in diestrus stage were subjected to ovariectomy and in 4-6 weeks they were given subcutaneously 17-beta-oestradiol (1 or 0.1 mkg per one animal). One hour before the animals were sacrificed, they were given 3H-thymidine intraperitoneally. It has been stated that 15-20 h after the estrogen administration the amount of DNA-synthesizing cells in the vaginal epithelium of these animals is 25 times as great as that in the control animals--castrated mice. When the epidermal chalone is administered locally or intraperitoneally (5 mg per one mouse), 10 min after the hormone injection, there is no inhibiting effect of the chalone on the proliferative processes in the vaginal epithelium stimulated with estrogen. When a single intraperitoneal injection of the epidermal chalone is given (5 mg per one mouse) 1 h before, or when it is given three times (2 mg per one mouse) 8, 4 or 1 h before the hormone is injected, there is a definite inhibiting effect in the proliferative processes. Their degree depends on how long the chalone was in contact with the cells.
对处于动情间期的非近交系和杂交小鼠、C57Bl品系和CBA品系小鼠进行卵巢切除，4至6周后给它们皮下注射17-β-雌二醇（每只动物1微克或0.1微克）。在处死动物前1小时，给它们腹腔注射3H-胸腺嘧啶核苷。据报道，雌激素给药后15至20小时，这些动物阴道上皮中DNA合成细胞的数量是对照动物（去势小鼠）的25倍。当在激素注射后10分钟局部或腹腔注射表皮抑素（每只小鼠5毫克）时，抑素对雌激素刺激的阴道上皮增殖过程没有抑制作用。当在激素注射前1小时单次腹腔注射表皮抑素（每只小鼠5毫克），或者在激素注射前8、4或1小时三次注射（每只小鼠2毫克）时，对增殖过程有明确的抑制作用。其抑制程度取决于抑素与细胞接触的时间长短。
