Ineichen K, Shepherd J C, Bickle T A
Nucleic Acids Res. 1981 Sep 25;9(18):4639-53. doi: 10.1093/nar/9.18.4639.
We have determined 3,400 base pairs of DNA sequence from the phage gamma genome which starts to the right of PL and runs to the left into the gene bet. The sequence thus includes the genes, N, ral, Ea10, cIII, kil and gam, as well as the transcription terminators TL1 and TL2. One surprising feature of the sequence is the presence in the region expected to be occupied by ral of a long open reading frame that, if it is expressed, would have to be transcribed from left to right, or counter to transcription from PL.
我们已经确定了噬菌体γ基因组3400个碱基对的DNA序列,该序列从PL右侧开始,向左延伸至基因bet。因此,该序列包括基因N、ral、Ea10、cIII、kil和gam,以及转录终止子TL1和TL2。该序列一个令人惊讶的特征是,在预期被ral占据的区域存在一个长的开放阅读框,如果它被表达,将不得不从左向右转录,即与从PL的转录方向相反。