Bernardi F, Bernardi A
Mol Gen Genet. 1984;195(3):452-8. doi: 10.1007/BF00341446.
The element IS102 potentially codes for two polypeptide chains. We have introduced several mutations in the larger one near the COOH terminus and determined the residual ability of the mutated elements to generate deletions in order to assign a role to this polypeptide chain. We show that in these elements, deletions still occur, although at a reduced level, but that in all cases examined so far the ends of the element are no longer recognized as the fixed endpoint of IS-mediated deletions, even though some other structural features of normal deletions formation are still present.
IS102元件可能编码两条多肽链。我们在较大的那条多肽链靠近COOH末端的区域引入了几个突变，并确定了突变元件产生缺失的剩余能力，以便确定该多肽链的作用。我们发现，在这些元件中，缺失仍然会发生，尽管频率降低了，但在目前所有检测的情况下，元件的末端不再被识别为IS介导缺失的固定端点，尽管正常缺失形成的一些其他结构特征仍然存在。
