Loviny T, Neuberger M S, Hartley B S
Biochem J. 1981 Feb 1;193(2):631-7. doi: 10.1042/bj1930631.
We have determined the nucleotide sequence of a secondary phage lambda attachment site (att) located between the structural genes of the ribitol and D-arabitol catabolic operons of Klebsiella aerogenes. The core region of this secondary attachment site (sequence: GGTTTTTTCGATTAT) shows considerable homology with the 15-base-pair core region common to both the phage att and the primary bacterial att of Escherichia coli K12 (sequence: GCTTTTTTACTAA); however, there is no such clear homology between the sequences flanking the cores of the primary att and this secondary att. Integration of phage lambda into the K. aerogenes secondary att occurred by recombination between the core region of the phage att and an oligo(T.A) stretch located within the K. aerogenes secondary att.
我们已经确定了位于产气克雷伯菌核糖醇和D -阿拉伯糖醇分解代谢操纵子结构基因之间的一个λ噬菌体二级附着位点（att）的核苷酸序列。这个二级附着位点的核心区域（序列：GGTTTTTTCGATTAT）与噬菌体att和大肠杆菌K12的主要细菌att共有的15个碱基对的核心区域（序列：GCTTTTTTACTAA）显示出相当高的同源性；然而，主要att核心两侧的序列与这个二级att核心两侧的序列之间没有如此明显的同源性。λ噬菌体整合到产气克雷伯菌二级att中是通过噬菌体att的核心区域与产气克雷伯菌二级att内的一段寡聚（T.A）序列之间的重组发生的。
