Brunner G, Lang K, Wolfe R A, McClure D B, Sato G H
Brain Res. 1981 Nov;254(4):563-75. doi: 10.1016/0165-3806(81)90024-9.
We cultured single cell suspensions derived from enzymatically digested rat and mouse brains in the serum-free, hormone-supplemented media originally established for culturing glioma (C6) and neuroblastoma cell lines (B104). We succeeded in selecting different cell types by varying the hormones added to the culture medium. In addition to the medium the attachment factors used for coating the culture dishes proved important for the establishment of a selective culture system. In a comparative morphological study we show that different particular microenvironmental conditions determine the growth and/or survival of different cell types of dissociated brain cells.
我们将通过酶消化大鼠和小鼠大脑获得的单细胞悬液，培养于最初为培养胶质瘤（C6）和神经母细胞瘤细胞系（B104）而建立的无血清、添加激素的培养基中。通过改变添加到培养基中的激素，我们成功地筛选出了不同的细胞类型。除了培养基之外，用于包被培养皿的贴壁因子对于建立选择性培养系统也很重要。在一项比较形态学研究中，我们发现不同的特定微环境条件决定了解离脑细胞不同细胞类型的生长和/或存活。
