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在合成培养基中对大鼠中枢神经系统神经元进行选择性培养。

Selective culture of rat CNS neurons in a synthetic medium.

作者信息

Ferraro D, Cestelli A, Barbieri R, Vitale F, Savettieri G

出版信息

Ital J Neurol Sci. 1983 Sep;4(3):323-6. doi: 10.1007/BF02043486.

DOI:10.1007/BF02043486
PMID:6642999
Abstract

A Chemically Defined Medium is described which facilitates the survival of neurons in primary cultures of rat cerebral hemispheres. More than 90% of all cells were identified as neurons using neurofilament as a marker in an immunocytochemical assay. In contrast, serum-supplemented medium, by stimulating nonneuronal cell proliferation, hinders the survival of neurons in culture.

摘要

本文描述了一种化学成分明确的培养基,该培养基有助于大鼠大脑半球原代培养物中神经元的存活。在免疫细胞化学分析中,使用神经丝作为标志物,超过90%的细胞被鉴定为神经元。相比之下,补充血清的培养基通过刺激非神经元细胞增殖,阻碍了培养物中神经元的存活。

相似文献

1
Selective culture of rat CNS neurons in a synthetic medium.在合成培养基中对大鼠中枢神经系统神经元进行选择性培养。
Ital J Neurol Sci. 1983 Sep;4(3):323-6. doi: 10.1007/BF02043486.
2
Formulation of a novel synthetic medium for selectively culturing rat CNS neurons.一种用于选择性培养大鼠中枢神经系统神经元的新型合成培养基的配方。
Brain Res. 1985 Oct;354(2):219-27. doi: 10.1016/0165-3806(85)90173-7.
3
A neurotrophic factor (NTF) released from primary glial cultures supports survival and fiber outgrowth of cultured hippocampal neurons.从原代神经胶质细胞培养物中释放的一种神经营养因子(NTF)可支持培养的海马神经元的存活和纤维生长。
J Neurosci Res. 1982;8(2-3):195-204. doi: 10.1002/jnr.490080209.
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Rat CNS cell culture. Enhancement of neuronal survival and delay of glial proliferation by serum from patients with multiple sclerosis. A morphological study.大鼠中枢神经系统细胞培养。多发性硬化症患者血清对神经元存活的增强作用及对胶质细胞增殖的延迟作用。一项形态学研究。
Ital J Neurol Sci. 1984 Sep;5(3):311-6. doi: 10.1007/BF02043964.
5
Immunocytochemical characterization of neuron-rich primary cultures of embryonic rat brain cells by established neuronal and glial markers and by monospecific antisera against cyclic nucleotide-dependent protein kinases and the synaptic vesicle protein synapsin I.通过已确立的神经元和神经胶质标志物以及针对环核苷酸依赖性蛋白激酶和突触小泡蛋白突触素I的单特异性抗血清,对胚胎大鼠脑细胞富含神经元的原代培养物进行免疫细胞化学表征。
Brain Res. 1986 Jan 22;363(2):205-21. doi: 10.1016/0006-8993(86)91006-1.
6
CNS neuronal cell line-derived factors regulate gliogenesis in neonatal rat brain cultures.中枢神经系统神经元细胞系衍生因子调节新生大鼠脑培养物中的神经胶质细胞生成。
J Neurosci Res. 1988 Jul;20(3):291-303. doi: 10.1002/jnr.490200303.
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[In vitro cytodifferentiation of isolated neuroblasts of chick embryo cerebral hemispheres: effects of the medium].[鸡胚大脑半球分离神经母细胞的体外细胞分化:培养基的作用]
J Physiol (Paris). 1971;63(6):284A-285A.
8
[The effect of a synthetic tripeptide nervous tissue cultured in vitro].
Z Mikrosk Anat Forsch. 1979;93(5):820-8.
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Growth requirements in vitro of oligodendrocyte cell lines and neonatal rat brain oligodendrocytes.少突胶质细胞系和新生大鼠脑少突胶质细胞的体外生长需求。
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1955-9. doi: 10.1073/pnas.83.6.1955.
10
Regulation of the proliferation of embryonic chick leptomeningeal cells in vitro by both contact inhibition and co-culture with CNS neurons.通过接触抑制以及与中枢神经系统神经元共培养对体外培养的胚胎鸡软脑膜细胞增殖的调节。
J Neurosci Res. 1982;8(2-3):523-34. doi: 10.1002/jnr.490080238.

本文引用的文献

1
Methods for growth of cultured cells in serum-free medium.在无血清培养基中培养细胞的方法。
Anal Biochem. 1980 Mar 1;102(2):255-70. doi: 10.1016/0003-2697(80)90151-7.
2
Simultaneous separation and purification of neurofilament and glial filament proteins from brain.从大脑中同时分离和纯化神经丝蛋白和神经胶质丝蛋白。
J Neurochem. 1982 Jan;38(1):142-50. doi: 10.1111/j.1471-4159.1982.tb10865.x.
3
Growth of purified astrocytes in a chemically defined medium.纯化星形胶质细胞在化学成分明确的培养基中的生长。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):7205-9. doi: 10.1073/pnas.78.11.7205.
4
Selective cell culture of brain cells by serum-free, hormone-supplemented media: a comparative morphological study.使用无血清、添加激素的培养基对脑细胞进行选择性细胞培养:一项比较形态学研究。
Brain Res. 1981 Nov;254(4):563-75. doi: 10.1016/0165-3806(81)90024-9.
5
Attachment and culture of dissociated cells from rat embryo cerebral hemispheres on polylysine-coated surface.大鼠胚胎大脑半球解离细胞在聚赖氨酸包被表面的贴壁与培养
J Cell Biol. 1974 Aug;62(2):540-6. doi: 10.1083/jcb.62.2.540.
6
A procedure for purifying neuron-like cells in cultures from central nervous tissue with a defined medium.
Dev Neurosci. 1979;2(5):233-7. doi: 10.1159/000112485.
7
Growth of a rat neuroblastoma cell line in serum-free supplemented medium.大鼠神经母细胞瘤细胞系在无血清补充培养基中的生长。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):514-7. doi: 10.1073/pnas.76.1.514.
8
Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.在添加激素的无血清培养基中培养和维持海拉细胞。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):901-4. doi: 10.1073/pnas.75.2.901.